Debao Biotech

Cardiac Troponin I (cTnI) is a protein found in cardiac muscle, and is part of a three subunit complex comprised of Troponin I, Troponin T and Troponin C. Along with tropomyosin, this structural complex forms the main component regulating the calcium sensitive ATPase activity of actomyosin in striated skeletal and cardiac muscle.

After cardiac injury, Troponin I is released into the blood 4-6 hours after the onset of chest pains. The release pattern of cTnI is similar to CK-MB, but while CK-MB levels return to normal after 72 hours, Troponin I remains elevated for 6-10 days, providing for a longer window of detection for cardiac injury. The high specificity of cTnI for the identification of myocardial damage has been demonstrated in conditions such as perioperative periods, after marathon runs, and blunt chest trauma.

cTnI release has also been documented in cardiac conditions other than acute myocardial infarction (AMI) such as unstable angina, congestive heart failure, and ischemic damage due to coronary artery bypass surgery. Because of its high specificity and sensitivity in the myocardial tissue, Troponin I has recently become the preferred biomarker for myocardial infarction.

Individually pouched test devices	Each test contains a strip with colored conjugates and reactive reagents precoated at the corresponding regions
Disposable pipettes	For adding specimens.
Buffer	4 mL/vial. Phosphate buffered saline and preservative
Package insert	Operating instructions

Bring tests, specimens, buffer, and/or controls to room temperature (15-30°C) before use.

1. Remove the test from its sealed pouch, and place it on a clean, level surface. Label the device with patient or control identification. For best results, the assay should be performed within one hour.

2. Using the disposable pipette provided in the kit, add 2 drops of serum or plasma to the specimen well (S) of the device, then start the timer.

Add 3 drops of whole blood specimen to the specimen well (S) of the device with the provided disposable pipette, then add 1 drop of buffer and start the timer.

Allow 3 hanging drops of fingerstick whole blood specimen to fall into the center of the specimen well (S) on the device, then add 1 drop of buffer and start the timer.

Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.

As the test begins to work, color will migrate across the membrane.

3. Wait for the colored band(s) to appear. The result should be read at 10 minutes. Do not interpret the result after 20 minutes.